Fig. 3

circ_0012381 from irradiated GBM cell-derived exosomes induced the M2 polarization of microglia and inhibited phagocytosis. A The massive circRNAs were significantly different in exosomes secreted by irradiated GBM cells compared with exosomes secreted by non-irradiated GBM cells. As well, compared with HMC3 cells that phagocytized exosomes secreted by non-irradiated GBM cells, a lot of circRNAs were significantly different in HMC3 cells which phagocytized exosomes from irradiated GBM cells. B The number of the overlapping upregulated circRNAs was 37 in 2 heat maps. C Upregulation of circ_0012381 was the most significantly in 37 circRNAs. D RT-PCR identified that radiation upregulated the level of circ_0012381 of U251 and HMC3 in co-cultuer system. E Circ_0012381 increased TGFβ, CCL2, IL-1, IL-10, and CD163 expression and decreased TNFα expression in HMC3 cells. F Circ_0012381 inhibited the phagocytic capability of HMC3 cells