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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: The potential of mecciRNA in hepatic stellate cell to regulate progression of nonalcoholic hepatitis

Fig. 6

Validation of mecciRNAs Networks in vitro and in vivo. A Under the stimulation of LPS (100 ng/ml, 48 h), LX-2 cells were significantly activated, and their cell morphology changed. Scale bar = 10 μm. B RT-qPCR result shows that the expression levels of hsa_circ_0087761 and hsa_circ_0087763 were decreased in activated LX-2 cells. The expression of GAPDH was used as the reference in the RT-qPCR analyses. **P < 0.01, ***P < 0.001. C RT-qPCR result shows that miR-642a-5p, miR-1248, miR-670-3p, and miR-1224-3p were upregulated significantly in activated LX-2 cells, while the expression level of miR-4667-3p was not changed. The expression of U6 was used as the reference in the RT-qPCR analyses. *P < 0.05, **P < 0.01, ***P < 0.001, n.s. means none was statistically significant. D, E Endogenous levels of SMAD2, SMAD3, and c-MYC from control or LPS-stimulated LX-2 cells (n = 3) were determined by Western blot (D). GAPDH was used as a loading control. The relative ratios of indicated proteins over GAPDH and statistical analysis results are shown (E). *P < 0.05, **P < 0.01, ***P < 0.001. F HE stained images shows that the mice in MCD-diet group had significant hepatic steatosis compared to the mice in standard diet group. Scale bar = 100 μm. G, H Endogenous levels of THBS1, STAT3, and p-STAT3 from control or MCD-diet mice (n = 6) were determined by Western blot (G). GAPDH was used as a loading control. The relative ratios of indicated proteins over GAPDH and statistical analysis results are shown (H). *P < 0.05, **P < 0.01, ***P < 0.001, n.s. means none was statistically significant

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