Skip to main content
Fig. 3 | Journal of Translational Medicine

Fig. 3

From: Low mitochondrial DNA copy number induces chemotherapy resistance via epithelial-mesenchymal transition by DNA methylation in esophageal squamous cancer cells

Fig. 3

Association between mtDNA and mitochondrial membrane potential. A The rate of transition from PE-A to FITC-A after 5 µM CDDP administration was lower in mtDNA-depleted ESCC cells than control cells, and the depolarization of mitochondrial membrane potential was significantly lower in mtDNA-depleted ESCC cells than control cells. B The ESCC cell line which was exposure CDDP for long term had lower the fluorescence of mtDNA and mitochondrial membrane potential (MMP) than control cell lines. Scale bar, 20 µM. Quantification of mtDNA and MMP fluorescence per cell (quantification/cell). C ESCC cells which was administrated CDDP for long time had lower fluorescence of mtDNA and MMP compared control cells. D There was lower fluorescence of mtDNA and MMP in mtDNA-depleted ESCC cells than control cells. On the other hand, mtDNA-increased ESCC cells had higher fluorescence of mtDNA and MMP than control cells. Scale bar, 20 µM. Quantification of mtDNA and MMP fluorescence per cell (quantification/cell). E, F The complex activity assay kit showed that mtDNA-depleted ESCC cells reduced complex I and IV activity assay compared to control cells. On the other hand, there was higher complex I and IV activity in mtDNA-increased ESCC cells than control cells

Back to article page