Table 3 Analysis of sequence characteristics of the ODAC construct. Physicochemical parameters, antigenicity, solubility and toxicity of the sequence were computed through Expasy’s ProtParam, ANTIGENpro/VaxiJen, SOLpro and Toxin Pred, respectively. Also, the allergenicity of the desired sequence was evaluated using AllerTOP and AlgPred
Protparam tool | Vaxi Jen | ToxinPred | Aller TOP | Alg Pred | Scratch protein predictor | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sequence Length | M.wt |  + R | − R | pI | Half-life in | II | AI | GRAVY | SOL pro | Antigen pro | ||||||
Mammalian | Yeast | E. coli | ||||||||||||||
372 | 41.93 | 60 | 75 | 5.29 | 30 h |  > 20 h |  > 10 h | 36.83 | 68.01 | -0.954 | 0.748 | Non toxin | Non allergen | Non allergen | 0.89 | 0.92 |