Table 2 Details of the chosen domains of PhtD, PsaA and PspC for designing the final construct
Protein | PhtD-C | PsaA | PspC |
|---|---|---|---|
Final selected domain | (Residues 196–256) AAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGS | (Residues 100–187) SDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSAKDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEG | (Residues 276–363) RRNYPSNTYFSLELEISESDVEVKKAEFELVKEEAKEPRNEEKVKQAKAKVESKKAVATRLENIKTDRKKAEEEAKRKAAEEDKVKEK |
Final B-cell epitopes | 224–238 225–239 226–240 234–248 237–251 238–252 239–253 240–255 241–256 | 103–117 104–118 105–119 106–120 107–121 108–122 109–123 110–124 113–127 133–147 141–155 148–162 | 285–299 288–302 294–308 295–309 324–339 327–341 |
Final MHC-II Epitopes (Mouse) | 189–203 190–204 217–231 218–232 223–237 224–238 225–239 226–240 227–241 | 174–188 175–199 176–190 | 288–302 289–303 290–304 298–312 299–313 300–314 301–315 316–330 333–347 323–337 322–336 321–335 324–338 320–334 |
Final MHC-II epitopes (Human) | 222–236 223–237 224–238 225–239 227–241 228–242 240–254 241–255 | 103–117 104–118 126–140 167–181 168–182 169–183 | 303–317 334–348 335–349 336–350 337–351 333–347 |