Fig. 8
H3K27ac mediated Sirt1 and HO1 action regarding CD48 expression. A HEK293T cells were transfected with the His-tagged HO1 and Flag-tagged Sirt1 plasmids. Cell lysates were immunoprecipitated with anti-His-HO1 or anti-Flag-Sirt1 antibody and immunoblotted with anti-Flag-Sirt1 or anti-His-HO1 antibody, respectively. B THP-1 cell lysates were immunoprecipitated with anti-HO1 or anti-Sirt1 antibody and immunoblotted with anti-Sirt1 or anti-HO1 antibody, respectively. C MV4-11 cell lysates were immunoprecipitated with anti-HO1 or anti-Sirt1 antibody and immunoblotted with anti-Sirt1 or anti-HO1 antibody, respectively. D Sirt1 deacetylation activity assay of Sirt1 immunoprecipitated from extracts of THP-1 expressing EV1 or LV-HO1. Proteins loaded were analyzed by western blotting. This experiment has been repeated for three times. E Sirt1 deacetylation activity assay of Sirt1 immunoprecipitated from extracts of MV4-11 expressing EV2 or Si-HO1. Proteins loaded were analyzed by western blotting. This experiment has been repeated for three times. F H3K27ac levels in EV1 and LV-HO1 THP-1 cells were quantified by western blotting analysis. LV-HO1 cells treated with selisistat are also shown in this graph. G H3K27ac levels in EV2 and Si-HO1 MV4-11 cells were quantified by western blotting analysis. Si-HO1 cells treated with SRT1720 are also shown in this graph. H CD48 mRNA levels in EV1 and LV-HO1 THP-1 cells were quantified by qRT-PCR analysis. LV-HO1 cells treated with selisistat are also shown in this graph. I CD48 mRNA levels in EV2 and Si-HO1 MV4-11 cells were quantified by qRT-PCR analysis. Si-HO1 cells treated with SRT1720 are also shown in this graph. Statistical differences were determined using the Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001