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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Lkb1 aggravates diffuse large B-cell lymphoma by promoting the function of Treg cells and immune escape

Fig. 1

Increased Treg abundance within the tumor tissue and PBMC of patients with DLBCL. A T cells from DLBCL (n = 4) and reactive lymph nodes (n = 3) biologically independent samples were combined and jointly visualized using t-SNE. The cells were coloured with respect to their cluster. B Proportion of Treg cells and Treg subpopulations identified in samples from the two group based on scRNA-seq in pie charts. C Immunofluorescence staining of Treg cells from different groups for Foxp3 (red) and DAPI (blue). D The quantification of immunofluorescence staining of Foxp3+ cells in lymphatic tissues from DLBCL patients and reactive lymph node. Frequencies of CD4+ CD25high CD127low/neg Tregs (E) and CD45RA− effector Tregs (F) in infiltrating lymphatic tissues of DLBCL patients (n = 3) and lymph node tissue from control group (n = 5). G Representative flow cytometric analysis of Tregs frequencies in PBMC sample from healthy donors (n = 27), DLBCL patients (n = 30) and the patients after treatment (n = 16). Within the CD4+ T cell gate, Tregs were identified as CD25high CD127low/neg. H Frequencies of Tregs in PBMC from healthy donors (n = 27), DLBCL patients (n = 30) and the complete remission (n = 11) and no remission (n = 5) patients after treatment. I Representative flow cytometric analysis of CD45RA− effector Tregs in each Treg subset frequencies in PBMC sample from healthy donors and DLBCL patients. The percentages of effector Tregs within the CD4+ T cell gate in PBMC from healthy donors (n = 11) and DLBCL patients (n = 13) were shown. *P < 0.05; **P < 0.01, ***P < 0.001. Asterisk indicates significant difference from control group as assessed. Pound sign indicates significant difference from different Treg group. In H, p values were determined by one-way ANOVA; In D–F and I, p values were determined by two-sided unpaired t-test; data are presented as mean values ± SEM

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