Fig. 5

miR-877-5p regulates the BCR-ABL expression. A Differential expression of miR-877-5p between CML and normal donors bone marrow samples. The efficiency of miR-877-5p inhibitor or mimic in K562 (B) and K562/G01 (C) cells were determined with RT-qPCR. Relative BCR-ABL mRNA (D) and protein (E) expression levels were detected by RT-qPCR with transfecting miR-inhibitor or miR-mimic, respectively. F, G Effect of miR-inhibitor or miR-mimic on viability in CML cells was measured with CCK-8 assays. H, I Colony formation assays were used for evaluating the effect of miR-inhibitor or miR-mimic on proliferation in CML cells. *p < 0.05, **p < 0.01, ***p < 0.001 and **** < 0.0001