Skip to main content
Fig. 4 | Journal of Translational Medicine

Fig. 4

From: circCRKL, a circRNA derived from CRKL, regulates BCR-ABL via sponging miR-877-5p to promote chronic myeloid leukemia cell proliferation

Fig. 4

BCR-ABL levels were downregulated in circCRKL silencing CML cells. Effect of circCRKL silencing on BCR-ABL protein levels was evaluated by western bolt assays (A) and immunofluorescence (B). Scale bar, 10 μm. C Effect of circCRKL knockdown on BCR-ABL mRNA levels in CML cells was determined with RT-qPCR. D The Venn diagram illustrating the overlapped miRNAs for circCRKL predicted using ENCORI, circInteractome, and circBank. E The Venn diagram showed the overlapped miRNAs between circCRKL targets and candidates for ABL obtained from ENCORI, TargetScan, and miRwalk. F The schematic diagrams of binding sites of circCRKL with miR-877-5p, and ABL 3’UTR with miR-877-5p. G, H Relative luciferase activities of the reporter plasmids were assessed in 293 T cells treated with miR-877-5p mimic or miR-NC. I Relative enrichment of circCRKL and miR-877-5p on circCRKL probe in K562/G01 cells was evaluated with RNA pulldown assay. J Relative enrichment of circCRKL and miR-877-5p on AGO2 protein in K562/G01 cells was detected by RNA immunoprecipitation. *p < 0.05 and ** < 0.01

Back to article page