Fig. 1

Culture, harvesting and identification of DU145 cell sheets. A: DU145 cell culture. Cells reached 90% confluence, manifested as polygonal or spindle shaped. Scale bar: 100 μm. B: DU145 cell sheet was observed under light microscopy. Cells were closely connected with white collagen. Scale bar: 100 μm. C: DU145 cell sheet in the 35 mm dish. D: SEM images of DU145 cell sheets. Scale bar: 100 μm. E: HE staining revealed that the DU145 cell sheets were composed of 3–5 layers of cells. F–I: Immunohistochemical staining was used to identify DU145 cell sheets. F: CK-8, G: vimentin, H: type I collagen, I: desmin. Scale bar: E–I, 50 μm