Fig. 3

In vitro cell cycle assay. A The diagram of cell cycle. B In HeLa cells, cell cycle experiments showed that SPOP knockdown could significantly promote the proportion of G1 phase cells and reduce the proportion of S and G2 phase cells. In SiHa cells, cell cycle experiments showed that SPOP knockdown could significantly promote the percentage of G1 phase cells and reduce the percentage of S phase cells. In ME-180 cells, cell cycle experiments showed that overexpression of SPOP could significantly reduce the proportion of G1 phase cells and increase the proportion of G2 phase cells. In MS751 cells, cell cycle experiments showed that overexpression of SPOP could significantly reduce the proportion of G1 phase cells and increase the proportion of S phase cells