Fig. 4

PCBP1 mediates DKK1 mRNA stability. A Graph based on the above results showing that DKK1 was the key gene. B qPCR analysis of the levels of DKK1 in PCBP1 knockdown and overexpression cells. C Western blot analysis of the levels of DKK1 in PCBP1 knockdown and overexpression cells. D The interaction of PCBP1 with DKK1 was examined by RIP-qPCR in A549 cells. E The interaction of PCBP1 with DKK1 was assessed by RNA pull-down assays followed by western blot analysis and silver staining. F mRNA expression of PCBP1-overexpressing cells and control cells treated with actinomycin D was examined by qPCR. *p < 0.05; **p < 0.01; ns, not significant