Fig. 6

GAS6-AS1 regulats TRIM14 via ceRNA network and FUS-dependent manner. A, B miR-370-3p/miR-1296-5p and TRIM14 binding sequences and TRIM14 mutation sequences. The luciferase activities in 293T cells co-transfected with wild-type (WT) or mutant (370Mut/1290Mut) TRIM14 plasmid together with miR-370-3p or miR-1296 mimic or miR-NC. C The mRNA level of TRIM14 in indicated CRC cells were evaluated by qRT-PCR. D The protein level of TRIM14, E-cadherin, N-cadherin and Vimentin in indicated CRC cells were evaluated by western blot. E The mRNA level of TRIM14 in indicated CRC cells were evaluated by qRT-PCR. F The protein level of TRIM14, E-cadherin, N-cadherin and Vimentin in indicated CRC cells were evaluated by western blot. G RIP assay verified the interaction of GAS6-AS1 and TRIM14 mRNA with FUS. H RIP assay evaluated the impact of GAS6-AS1 on FUS-interacted TRIM14 mRNA. I qRT-PCR detected the expression of TRIM14 in indicated CRC cells. J TRIM14 expression after actinomycin D treatment for indicated times was tested by qRT-PCR. * P < 0.05, ** P < 0.01