Fig. 4

GAS6-AS1 serves as a ceRNA and sponges miR-370-3p and miR-1296-5p. A Subcellular localization of GAS6-AS1 was detected by qRT-PCR in LoVo cells. B Subcellular localization of GAS6-AS1 in HT29 and LoVo cells determined by RNA-FISH (scale bar: 20 μm). C Fold enrichment of GAS6-AS1 in LoVo. D Enrichment of GAS6-AS1 in LoVo cells transfected with miR-370-3p mimic, miR-1296-5p mimic or miR-NC. E Ago2 protein immunoprecipitated by Ago2 antibody or IgG was meatured by western blot. F miR-370-3p/miR-1296-5p and GAS6-AS1 binding sequences and GAS6-AS1mutation sequences. G The luciferase activities in 293T cells co-transfected with wild-type (WT) or mutant (370Mut/1290Mut) GAS6-AS1 plasmid together with miR-370-3p or miR-1296-5p mimic or miR-NC. H qRT-PCR showed that miR-370-3p and miR-1296-5p level were decreased when GAS6-AS1 overexpressed. I qRT-PCR showed that miR-370-3p and miR-1296-5p level were upregulated when GAS6-AS1 knockdown. ** P < 0.01, *** P < 0.001