Fig. 4From: IGF2BP1-regulated expression of ERRα is involved in metabolic reprogramming of chemotherapy resistant osteosarcoma cellsThe m6A methylated 3′UTR of ERRα was critical for its mRNA stability. The m6A methylation of ERRα mRNA in parental or Dox-resistant MG-63 (a) and HOS (b) cells were checked by m6A-RIP-PCR; the m6A enrichment of ERRα mRNA in MG-63 (c) or HOS (d) cells were checked by m6A-RIP-qPCR and fragmented RNA; e, f The m6A methylation of 3′UTR of ERRα mRNA in parental or Dox-resistant MG-63 (e) and HOS (f) cells; g the schematic diagram for luciferase reporter by use of pmirGLO and CDS or 3′UTR of ERRα mRNA; h MG-63 or MG-63/Dox cells were transfected with pmirGLO-CDS or pmirGLO-3′UTR for 24 h, and then the relative levels of F-Luc/R-Luc were checked; MG-63 or MG-63/Dox cells were transfected with pmirGLO-CDS (i) or pmirGLO-3′UTR (j) for 24 h and further incubated with Act-D for different time periods, then the mRNA of F-Luc in cells was checked by qRT-PCR. Data are presented as means ± SD from three independent experiments. **p < 0.01 by Student’s t testBack to article page