Fig. 8
From: The BRD4 inhibitor JQ1 suppresses tumor growth by reducing c-Myc expression in endometrial cancer
Overexpression of BRD4 reversed the effect of JQ1. A Protein expressions of BRD4 and β-Actin in Ishikawa-NC and Ishikawa-BRD4 overexpression cells were determined by western blot assay. β-Actin served as the loading control. B Ishikawa-NC and Ishikawa-BRD4 overexpression cells were treated with 0–10 µM JQ1 for 48 h. The cell viability of JQ1 was measured using MTT assay. C Cells were stained with Annexin V/FITC and PI, and then cell apoptosis was measured by flow cytometry. Data are shown in the histogram. Data shown are mean ± SD from three independent experiments