Fig. 6

BET inhibitors suppressed the cell cycle of EC cells in G1 phase in ishikawa cells. A Ishikawa cells were treated with DMSO, 2.5 µM, 5 µM and 10 µM JQ1 for 24 h. Cells were stained with PI and analyzed by flow cytometry. Data are shown in the histogram. Data shown are mean ± SD from three independent experiments. B Ishikawa cells were treated with JQ1 (0, 2.5, 5 and 10 µM) for 24 h (left) or 10 µM JQ1 for indicated times (right). Protein expressions of CDC25A, CDK4, CyclinD1, P21, phosphorylated Rb, Rb and β-Actin were determined by western blot assay. β-Actin served as the loading control. C Ishikawa cells were treated with OTX015( 0, 2, 4 and 8 µM) for 24 h. Cells were stained with PI and analyzed by flow cytometry. D Ishikawa cells were treated with indicated concentrations of OTX015 for 24 h. Protein expressions of CDC25A, CDK4, CyclinD1, P21, phosphorylated Rb, Rb and β-Actin were determined by western blot assay. β-Actin served as the loading control