Fig. 5
From: The BRD4 inhibitor JQ1 suppresses tumor growth by reducing c-Myc expression in endometrial cancer
JQ1 blocked PI3K/AKT/mTOR pathway in EC cells. A HEC-1A and Ishikawa cells were treated with DMSO, 2.5 µM, 5 µM and 10 µM JQ1 for 48 h. B HEC-1A and Ishikawa cells were treated with 10 µM JQ1 for indicated times. Protein expressions of PI3K, AKT, phosphorylated AKT, mTOR, phosphorylated mTOR and β-Actin were determined by western blot assay. β-Actin served as the loading control. C and D The relative expression levels of indicated proteins were measured by western blot analysis. β-Actin served as the control