Fig. 7

Asprosin promotes p38 and Elk-1 phosphorylation and ABCA1 and ABCG1 expression in the aortas. A Protein samples were immunoblotted with antibodies against p-p38, p38 or β-actin in the aortas (n = 5 mice per group). B Measurement of aortic Elk-1 expression by qRT-PCR and western blot (n = 5 mice per group). C Detection of aortic Elk-1 phosphorylation at Ser383 using western blot (n = 5 mice per group). D Western blot analysis of aortic Elk-1 protein levels in the cytoplasm and nucleus (n = 5 mice per group). E The qRT-PCR and western blot assays of ABCA1 and ABCG1 expression in the aortas (n = 5 mice per group). The results are shown as the mean ± SD. **P < 0.01, ***P < 0.001. NS indicates not significant