Fig. 2

Asprosin promotes ABCA1 and ABCG1 expression and cholesterol efflux from macrophages. A–E THP-1 macrophage-derived foam cells were transduced with LV-Mock or LV-Asprosin for 72 h (n = 3). A The expression of ABCA1 and ABCG1 was determined by qRT-PCR and western blot. B, C Representative fluorescent images of NBD-cholesterol burden and quantitative analyses of cholesterol efflux to apoA-I and HDL. D The qRT-PCR and western blot assays of CD36 and SR-A expression. E Representative fluorescent images and quantification of Dil-ox-LDL uptake. Data presented are mean ± SD. ***P < 0.001. NS indicates not significant