Fig. 2
solTNF induces MAPKi resistance in BRAFV600E+ melanoma cell lines that co-express TNFR1 and TNFR2. A BRAF.V600E+ SK-Mel-38 and SK-Mel-28 melanoma cell lines were cultured alone or with rhTNF (TNF) for 48 h. Some of the cells stimulated with rhTNF were co-cultured in the presence of selective solTNF inhibitor (INB03; DN-TNF) or non-selective TNF-blocking antibody (αTNF). B Calculated loss of sensitivity to MAPKi in response to rhTNF [% Loss of MAPKi sensitivity (media vs TNF)] was correlated to TNFR1, TNFR2 or CD271 mean fluorescent intensity (MFI) values measured in Fig. 1. Experiments in (A) and (B) were performed in quadruplicates. Data shown represent mean values and whiskers represent standard error. *p ≤ 0.05