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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: Interplay of Helicobacter pylori, fibroblasts, and cancer cells induces fibroblast activation and serpin E1 expression by cancer cells to promote gastric tumorigenesis

Fig. 6

Serpin E1 overexpression promotes the growth of xenograft tumors via inducing neovascularization in nude mice. A In vivo tumor formation assay shows that the xenograft tumors with Serpin E1-overexpression display faster growth than control tumors (NC). B Growth curve of xenograft tumors by measuring the tumor volume (V). V = 1/2 × length × width2 (mm3). * P < 0.05, ***P < 0.001, and **** P < 0.0001. C Weight of xenograft tumors removed from nude mice. **** P < 0.0001. D HE staining and IHC analysis for Ki 67, Serpin E1, and CD31 in xenograft tumors removed from nude mice. Scale bar = 500 μm. The blue square area is enlarged to the right of each image. Scale bar: 500 μm and 50 μm in the original and enlarged images, respectively. Dot-plot graph shows the quantification of IHC staining for Ki67, Serpin E1, and CD31. IHC scores = intensity score × percentage score. Data are presented as means ± SD from five independent samples. **** P < 0.0001. E Matrigel tube formation assay of HUVECs co-cultured with Serpin E1-overexpressed gastric cancer cells using the Transwell co-culture system. HUVECs were seeded in the lower chamber and gastric cancer cells in the upper chamber of the Transwell insert. GC represents primary gastric cancer cells. Scale bar = 100 μm. F Matrigel tube formation assay of HUVECs treated with human recombinant Serpin E1 (recSerpin E1) at a concentration of 2 ng/ml. Scale bar = 100 μm

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