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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: Transplanted allogeneic cardiac progenitor cells secrete GDF-15 and stimulate an active immune remodeling process in the ischemic myocardium

Fig. 4

Role of GDF15 in cardiac recovery. Basal protein expression of GDF15 in rat CPCs and secretome (A), GDF15 protein expression in rCPCsGDF15KD and rCPCsscramble (B). Cardiac functional parameters were measured after intramyocardial injection of one million rCPCs, rCPCsGDF15KD and placebo separately in the BN rat MI model. C LV ejection fraction, D LV fractional shortening. Representative picture (E) and quantification (F) of Masson trichrome staining in rat hearts. Serum ELISAs for the cytokines IL-10, IL-2, and TNF- α are shown in G, Single-cell suspensions of total heart lysates were used for flow cytometric analysis 5 days after intramyocardial injection of one million rCPCs, rCPCSGDF15KD, or placebo in BN rats. Flow images (H) and quantitative flow results for Tregs (I). Flow images (J) and quantitative flow results (K) for M2 cells. Phosphorylated NF-κB p65 flow images (L) and quantification (M). Whole hearts were also obtained on day 5 for immunohistochemistry studies. Images of phosphorylated NF-κB, FoxP3+, and DAPI staining (N) and quantification (O). Immunohistochemistry of phosphorylated NF-κB and sarcomeric actin with DAPI (P). Numerical data are summarized as box and whisker plots with a median value (black bar inside box), 25th and 75th percentiles (bottom and top of box, respectively), and minimum and maximum values (bottom and top whisker, respectively). The number (n) of rats in each group indicated near the (up/below/on) each respective box and whisker plot. Q Invitro co-culture assay with rCPCs/ rCPCSGDF15KD with BN rat spleenocytes.for 5 day and CD48 measured by FACS

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