Fig. 1

Comprehensive characterization of subclonal architecture of the 93 driver genes. A The percentage of patients carrying subclonal, clonal, and clonal-subclonal mutations in each gene. B The clonality of frequently altered genes in CRC. Each column represents a CRC case carrying at least 1 mutation in any of the studied genes. The bar plot on the right represents the number of times at which each mutation is observed in all mutated cases. C The distributions of cancer cell fraction (CCF) values for somatic events within CRC driver genes. The dark-blue dot represents clonal mutation for a driver gene. The pale-blue dot represents subclonal mutation for a driver gene. D Significant enrichment of CRC driver genes with clonal and subclonal mutations. The y-axis represents clonal ratio (c1/s1) and subclonal ratio (s1/c1) for each driver gene. c1 represents the number of clonal mutations in the driver gene; s1 represents the number of subclonal mutations in the driver gene. Q value (i.e. FDR-adjusted P-value) was obtained using the permutation test. The names of driver genes significantly enriched with clonal or subclonal alterations are labeled in red