Fig. 1

MiR-1307-3p plays oncogenic roles in GC progression. A Luciferase reporter assays for screening of miRNAs that potentially regulate circDIDO1. B Prediction of binding sites in circDIDO1 for miR-1307-3p. C HGC-27 cells were co-transfected with miR-1307-3p and wild-type (WT) or mutant (MUT) luciferase reporter vector for circDIDO1. The luciferase activity was determined as indicated. D RNA immunoprecipitation (RIP) assay for the binding of circDIDO1 to Ago2 protein. E Analysis of miR-1307-3p expression in tumor tissues and adjacent normal tissues from GC patients by using TCGA data. F qRT-PCR analyses of circDIDO1 expression in control and miR-1307-3p overexpressing GC cells. G–J Cell growth curve, colony formation, transwell migration, and matrigel invasion assays for control and miR-1307-3p overexpressing GC cells. Data are shown as mean ± SD (n = 3, **P < 0.01, *P < 0.05). Scale bars = 100 μm