Fig. 2

Knockdown of FOXS1 inhibits the biological behavior, apoptosis resistance, and EMT in CRC. A PCR and western blot were used to evaluate the knockout efficiency of the FOXS1-specific small interfering RNAs (siRNAs) (siFOXS1-1, siFOXS1-2, and siFOXS1-3) in LoVo and SW620 cells. B CCK-8, Colon formation (C) and (D) Edu assays were used to evaluate the proliferation of LoVo and SW620 cells after transfection with siFOXS1 for 48 h. E Cell mobility analyzed using a wound healing assay. F Migration and invasion of siFOXS1#3 and Control cells were detected using Transwell migration and invasion assays. G Propidium iodide (PI) and Annexin V staining levels were determined by flow cytometry to assess cell apoptosis in LoVo and SW620 cells transfected with siFOXS1 and Control. H Western blot analysis of E-cadherin, N-cadherin, vimentin, MMP9 protein expression in LoVo and SW620 cells transfected with FOXS1-siRNA. Results were shown as mean ± SD of three independent experiments, each experiment was performed in triplicate. *P < 0.05; **P < 0.01