Fig. 3

Glutamine depletion inhibited E-cadherin expression in CRC cells. A Western blot analysis showed that the protein levels in 1.0 mM and 0.5 mM glutamine groups decreased compared with normal glutamine groups in SW480 and DLD1 cells. The relative integrated density was 0.71 ± 0.04 in SW480 1.0 mM, 0.83 ± 0.05 in SW480 0.5 mM glutamine group; 0.79 ± 0.03 in DLD1 1.0 mM glutamine group, 0.52 ± 0.12 in DLD1 0.5 mM glutamine group, *P < 0.05; B immunostaining results showed that E-cadherin expression was decreased in 1.0 mM glutamine group compared with normal glutamine group in SW480 cells (×200)