Whole-exome sequencing identified mutational profiles of urothelial carcinoma post kidney transplantation

Table 2 Summary of whole-exome sequence analysis of human urothelial carcinomas in kidney transplantation (UCKT) and hemodialysis (UCHD)

		UCKT (N = 7)	UCHD (N = 5)
	Average age at diagnosis (years)	55	61.6
	Mean coverage	81	77
Sequencing results	Targeted bases with at least 10 reads (%)	96.19	95.78
	Known SNPs identified in targeted region^*	4198	2574
Somatic mutation	Total somatic mutations *	18,703	11,060
	Insertion or deletion	271	188
	Single based substitution	18,432	10,872
	Synonymous	1976	65
	Missense	13,980	8782
	Nonsense	948	550
	Frameshift	288	186
	Splice acceptor	904	923
	Splice donor	301	331
	Nonstop	35	35
Frequency (%) **	C > A	7.03	6.72
	C > G	7.74	6.78
	C > T	27.69	23.96
	T > A	40.03	44.63
	T > C	11.80	12.06
	T > G	5.70	5.86

The WES results of the 12 samples were aligned to the human reference genome (hg19) and further subjected for bioinformatics analysis. UCKT; N = 7 and UCHD; N = 5
*The number was calculated as the summation of the samples in each group
**The number was calculated as the average of the samples in each group

ISSN: 1479-5876