Fig. 4
From: SOX21-AS1 activated by STAT6 promotes pancreatic cancer progression via up-regulation of SOX21
SOX21-AS1 interacted with USP10 to deubiquitinate and stabilized SOX21 protein. A SOX21 protein level in sh-SOX21-AS1 transfected PC cells upon CHX treatment. B SOX21 protein level in sh-SOX21-AS1 transfected PC cells upon MG132 treatment. C Ubiquitination of SOX21 protein in two PC cells transfected with SOX21-AS1-shRNA. D Biotinylated sense and antisense SOX21-AS1 were transcribed in vitro a for RNA pull down assays. A specific band at about 87 kDa was excised after sliver staining. E Immunoblotting indicated specific association of USP10 and SOX21-AS1. F, G RIP assays confirmed the combination between USP10 and SOX21-AS1/SOX21. H Down-regulation efficiency of USP10 in two PC cells was verified via RT-aPCR and western blot. I SOX21 protein level in sh-USP10 transfected PC cells upon CHX treatment. J SOX21 protein level in sh-USP10 transfected PC cells upon MG132 treatment. K Ubiquitination of SOX21 protein in two PC cells transfected with USP10-shRNA. **P < 0.01