Fig. 4

Proteomic analysis and transcriptome sequencing. A Cluster analysis of differentially expressed genes indicated that the expression of multiple genes changed with up/downregulation of NCAPG (red indicates high gene expression, blue indicates low gene expression). B Enrichment analysis with DisGeNET suggested that abnormal expression of NCAPG was indeed closely correlated with liver-related tumor diseases (the size of the dot represents the number of genes annotated to the DisGeNET term, and the color from red to purple represents the significance of enrichment). C The KEGG enrichment analysis results indicated that there was a significant correlation between the NCAPG gene and the PI3K-AKT pathway (the size of each dot in the figure represents the number of genes annotated to the KEGG pathway, and the colour from red to purple represents increasing significance of enrichment). D Volcano plot showing that 3549 genes were upregulated and 3114 genes were downregulated when NCAPG was upregulated and that 451 genes were upregulated and 470 genes were downregulated when NCAPG was knocked down. E Venn diagram showing 618 differentially expressed genes. F Proteomic analysis indicated that CKII was one of five genes involved in the participation of NCAPG in protein phosphorylation