Fig. 3

NCAPG promotes the proliferation of liver cancer cells in vivo. A The weights of tumours from mice injected with MHCC97H cells transfected with NCAPG overexpression and corresponding control plasmids (pcDNA3.1( +)-NCAPG) and pcDNA3.1( +), respectively) showed a positive correlation with the expression of NCAPG. B The weights of tumours from mice injected with MHCC97H cells transfected with the NCAPG knockdown plasmid (shNCAPG) and shNC showed a positive correlation with the expression of NCAPG. C, D and E Immunohistochemical analysis and HE staining of tissues from mice in the NCAPG overexpression group and NCAPG knockdown group showing that NCAPG can promote tumour proliferation. Quantitative analysis showed the same result. F The FISH assay of samples from mice in the NCAPG overexpression group and NCAPG knockdown group showed that tumour proliferation was positively correlated with the expression of NCAPG (**P < 0.01, ***P < 0.001)