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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: NCAPG promotes the proliferation of hepatocellular carcinoma through the CKII-dependent regulation of PTEN

Fig. 2

NCAPG promotes the proliferation of liver cancer cells in vitro. A NCAPG protein expression in HCC cells was detected by western blotting after transfection with the NCAPG overexpression plasmids (pcDNA3.1( +)-NCAPG) and pcDNA3.1( +). Quantitative analysis showed the same pattern. B CCK-8 assays showed that upregulation of NCAPG significantly increased the proliferation of MHCC97H and HCCLM3 cells (***P < 0.001). C and D EdU incorporation and plate colony formation assays showed that upregulation of NCAPG significantly increased the proliferation of MHCC97H and HCCLM3 cells (***P < 0.001). E Western blotting was used to detect the expression of NCAPG and PCNA in cells transfected with the NCAPG knockdown plasmid (shNCAPG) and shNC. Quantitative analysis showed the same pattern. F, G and H CCK-8, EdU incorporation and plate colony formation assays were used to detect the effects of NCAPG knockdown on cell proliferation, and NCAPG knockdown in cells was found to significantly decrease cell proliferation (*P < 0.05, **P < 0.01, ***P < 0.001; independent Student’s t test)

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