Fig. 1

NCAPG is highly expressed in liver cancer tissues and is associated with poor prognosis. A The results from analysis of the three datasets selected from the Oncomine database (Dataset 1, Wurmbach Liver; Dataset 2, Roessler Liver 1; Dataset 3, Roessler Liver 2; ****P < 0.0001; independent Student’s t test) showed that NCAPG mRNA was overexpressed in HCC tumour tissues compared to adjacent nontumor tissues. B and C Western blot analysis of NCAPG in HCC tissues and adjacent nontumor tissues suggested that the protein levels of NCAPG were higher in tumour tissues. Quantitative analysis showed the same pattern (T, tumour; NT, nontumor). D NCAPG mRNA expression was examined in 85 pairs of primary liver cancer tissues and the corresponding normal adjacent tissues via qRT–PCR, which indicated that NCAPG mRNA was overexpressed in HCC tumour tissues compared to adjacent nontumor tissues. E Overall survival curves for two groups of patients with HCC: as NCAPG expression increased, patient prognosis worsened (Guichard Liver). F Immunohistochemical staining showed that NCAPG protein expression was higher in tumour tissue. Representative cases are shown (200 ×) (**P < 0.01, ***P < 0.001)