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Fig. 7 | Journal of Translational Medicine

Fig. 7

From: LINC00665 knockdown confers sensitivity in irradiated non-small cell lung cancer cells through the miR-582-5p/UCHL3/AhR axis

Fig. 7

LINC00665 augmented the immune escape of NSCLC cells through the miR-582-5p/UCHL3 regulatory axis by mediating the stability of AhR protein. A, Representative images of xenografts in tumor-bearing mice under irradiation treatment and injected with PC9 cells overexpressing LINC00665 alone or in combination with silencing AhR. B, Tumor weight of the xenografted tumor in tumor-bearing mice under irradiation treatment and injected with PC9 cells overexpressing LINC00665 alone or in combination with silencing AhR. C, qRT-PCR determination of miRNA levels of LINC00665, miR-582-5p, UCHL3, AhR, and PD-L1 in tumor tissues from tumor-bearing mice under irradiation treatment and injected with PC9 cells overexpressing LINC00665 alone or in combination with silencing AhR. D, Western blot analysis of protein levels of UCHL3, AhR, and PD-L1 in tumor tissues from tumor-bearing mice under irradiation treatment and injected with PC9 cells overexpressing LINC00665 alone or in combination with silencing AhR. E, Detection of ki67-positive cells was to assess tumor cell proliferation in tumor tissues from tumor-bearing mice under irradiation treatment and injected with PC9 cells overexpressing LINC00665 alone or in combination with silencing AhR. F, Cell apoptosis in tumor tissues from tumor-bearing mice under irradiation treatment and injected with PC9 cells overexpressing LINC00665 alone or in combination with silencing AhR determined by TUNEL staining. G, Representative immunofluorescence images of CD8, PD-1, D240 and PD-L1 in tumor tissue sections (the left panels) and corresponding statistical analysis (the right panel). H, I, ELISA detection of INF-γ (H) and TNF-α (I) in tumor tissues from tumor-bearing mice under irradiation treatment and injected with PC9 cells overexpressing LINC00665 alone or in combination with silencing AhR. J, qRT-PCR measurement of CXCL10 expression in tumor tissues from tumor-bearing mice under irradiation treatment and injected with PC9 cells overexpressing LINC00665 alone or in combination with silencing AhR. n = 10. * p < 0.05 versus the mice treated with PC9/Vector; # p < 0.05 versus the mice treated with PC9/Vector + Gy; & p < 0.05 versus the mice treated with PC9/LV-LINC00665 + sh-NC + Gy

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