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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: LINC00665 knockdown confers sensitivity in irradiated non-small cell lung cancer cells through the miR-582-5p/UCHL3/AhR axis

Fig. 4

LINC00665 functioned as a sponge of miR-582-5p and thus up-regulated the expression of UCHL3. A, Venn diagram of the UCHL3 binding miRNAs predicted by TargetScan and ENCORI databases, respectively, and the poorly expressed miRNAs in lung cancer samples in the GSE15008 and GSE48414 datasets. B, The expression of miR-582-5p in NSCLC samples (n = 187) and normal samples (n = 188) in the GSE15008 dataset. C, The expression of miR-582-5p in NSCLC samples (n = 154) and normal samples (n = 20) in the GSE48414 dataset. D, qRT-PCR measurement of miR-582-5p expression in tumor tissues and adjacent normal tissues from NSCLC patients (n = 45). E, qRT-PCR measurement of miR-582-5p expression in NSCLC cells. F, Binding of miR-582-5p to UCHL3 verified by dual luciferase reporter assay. G, Binding of miR-582-5p to UCHL3 verified by RNA pull-down assay. H, qRT-PCR measurement of the transfection efficiency of miR-582-5p mimic and miR-582-5p inhibitor in NSCLC cells; I, qRT-PCR measurement of UCHL3 expression in NSCLC cells in response to miR-582-5p mimic or miR-582-5p inhibitor. J, Venn diagram of the miR-582-5p binding lncRNAs predicted by the LncBase database and the highly expressed lncRNAs in lung cancer tissues in TCGA-LUAD and in TCGA-LUSC datasets. K, The expression of LINC00665 in LUAD samples (n = 526) and normal samples (n = 59) in the TCGA-LUAD dataset. L, The expression of LINC00665 in LUSC samples (n = 501) and normal samples (n = 49) in the TCGA-LUSC dataset. M, The expression of LINC00665 in radiotherapy-resistant H1299 lung cancer cell lines (n = 21) as well as radiotherapy-sensitive H460 lung cancer cell lines (n = 21) in the GSE20549 dataset. N, qRT-PCR measurement of LINC0065 expression in tumor tissues and adjacent normal tissues from NSCLC patients (n = 45). O, qRT-PCR measurement of LINC00665 expression in NSCLC cells. P, Binding of LINC00665 to miR-582-5p verified by dual luciferase reporter assay. Q, Binding of LINC00665 and miR-582-5p to Ago2 in A549 and PC9 cells verified by RIP assay. R, Binding of LINC00665 to miR-582-5p in A549 and PC9 cells determined by RNA pull-down assay. S, qRT-PCR measurement of miR-582-5p expression A549 cells treated with sh-LINC00665 or in PC9 cells treated with LV-LINC00665. T, qRT-PCR measurement of UCHL3 expression in A549 cells treated with sh-LINC00665 or combined with miR-582-5p inhibitor or in PC9 cells treated with LV-LINC00665 or combined with miR-582-5p mimic. * p < 0.05. Each cellular experiment was repeated 3 times

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