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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: LINC00665 knockdown confers sensitivity in irradiated non-small cell lung cancer cells through the miR-582-5p/UCHL3/AhR axis

Fig. 3

UCHL3 reduced the radiosensitivity of NSCLC cells by stabilizing AhR protein. A, Cell viability in A549 cells silencing UCHL3 and in PC9 cells overexpressing UCHL3 measured by CCK-8 assay. B, Colony formation potential of A549 cells treated with sh-UCHL3 or in PC9 cells treated with LV-UCHL3 measured by colony formation assay. C, Cell invasion in A549 cells treated with sh-UCHL3 or in PC9 cells treated with LV-UCHL3 measured by Transwell assay. D, Detection of γH2ax fluorescence in A549 cells treated with sh-UCHL3 or in PC9 cells treated with LV-UCHL3. E, Flow cytometry analysis of cell apoptosis in A549 cells treated with sh-UCHL3 or in PC9 cells treated with LV-UCHL3. F, Flow cytometry analysis of cell cycle distribution in A549 cells treated with sh-UCHL3 or in PC9 cells treated with LV-UCHL3. G, Cell viability in A549 cells treated with sh-UCHL3 or combined with LV-AhR or in PC9 cells treated with LV-UCHL3 or combined with sh-AhR measured by CCK-8 assay. H, Colony formation potential of A549 cells treated with sh-UCHL3 or combined with LV-AhR or in PC9 cells treated with LV-UCHL3 or combined with sh-AhR measured by colony formation assay. I, Cell invasion in A549 cells treated with sh-UCHL3 or combined with LV-AhR or in PC9 cells treated with LV-UCHL3 or combined with sh-AhR measured by Transwell assay. J, Detection of γH2ax fluorescence in irradiation-treated A549 cells treated with sh-UCHL3 or combined with LV-AhR or in PC9 cells treated with LV-UCHL3 or combined with sh-AhR. K, Flow cytometry analysis of cell apoptosis in A549 cells treated with sh-UCHL3 or combined with LV-AhR or in PC9 cells treated with LV-UCHL3 or combined with sh-AhR. L, Flow cytometry analysis of cell cycle distribution in A549 cells treated with sh-UCHL3 or combined with LV-AhR or in PC9 cells treated with LV-UCHL3 or combined with sh-AhR. *, # p < 0.05. Each cellular experiment was repeated 3 times

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