Fig. 4

sCCR2 reduces inflammatory cytokines in knee joint. A Knee joint tissues from Mock and sCCR2-E3, sCCR2-Full rat were immunohistochemically stained with specific antibodies to IL-1β, IL-6 and MMP13. B Numbers of positive cells were counted in high-power fields (magnification: ×400) with the aid of NIH ImageJ software and averaged for three randomly selected fields per tissue section. Bars show the mean ± SEM results in three rats per group. C MSCs of OA patients were transfected Mock or sCCR2-E3. After 6 h, the media was changed and the cells stimulated with LPS 100 ng/mL and MCP-1 10 ng/mL for 12 h. The mRNA level of SOX9, MMP1 and MMP3 were determined using real-time. The data show the mean ± SEM of three independent experiments. (**p < 0.01, *** p < 0.001)