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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: Spatial transcriptomics atlas reveals the crosstalk between cancer-associated fibroblasts and tumor microenvironment components in colorectal cancer

Fig. 6

Workflow of bioinformatic analysis. Two types of datasets were utilized in this project. ST data was used to explore the role of CAFs in TME within patients with colorectal cancer, while bulk RNA-seq dataset was applied to validate the clinical translational value of CAFs identified in ST data. Then immunofluorescence staining and immunohistochemical was applied to validate the results based on bioinformatics analysis and we confirmed the functions of two CAFs types in CRC

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