Fig. 4

Chemotherapy alters the tumor microenvironment. A, B UMAP plots show patient-specific spatial transcriptomics (ST) expression patterns between colon1 and colon2, A UMAP plot of ST data after CCA, B UMAP plot of ST data without CCA. C Cell compositions predicted in different patients by ssGSEA, predicted cell compositions in all clusters (left), predicted cell composition in fibroblast cluster (middle), predicted cell composition in tumor cluster (right). D Boxplots show the cell compositions of iCAFs, NK cells and monocytes in all clusters. E, F Box plots show the patient-specific metabolism patterns scored by ssGSEA. p value < 0.05 was considered as cut-off criteria