Fig. 5

MiR-761 and FOXM1 were the downstream target of circ-ARAP2. A The luciferase activity of circ-ARAP2 in HEK293T cells transfected with different miRNA mimics, which are putative binding sites for the circ-ARAP2 sequence. Luciferase activity was normalized by Renilla luciferase activity. B Prediction of binding sites of miR-761 in circ-ARAP2. The MUT version of circ-ARAP2 is presented. C Relative luciferase activity determined 48 h after transfection of HEK293T cells with miR-761 mimic/NC or circ-ARAP2 WT/Mut. Data are presented as means ± SD. ***p < 0.001. D Prediction of binding sites of miR-761 within the 3'UTR of FOXM1. The MUT version of 3'-UTR-FOXM1 is shown. E Relative luciferase activity determined 48 h after transfection of HEK293T cells with miR-761 mimic/NC or 3'UTR-FOXM1 WT/Mut. Data are presented as means ± SD. ***p < 0.001. F–K RT-qPCR detection showing the expression of circ-ARAP2, miR-761 and FOXM1. Data are presented as mean ± SD; ^*p < 0.05, ^***p < 0.001 vs. NC; ^##p < 0.01, ^###p < 0.001 vs. si-circ-ARAP2