Fig. 3

Downregulation circ-ARAP2 suppressed ESCC proliferation and tumor growth in both in vitro and in vivo. A RT-qPCR detection show the expression of circ-ARAP2 after transfected with siRNA against circ-ARAP2 (si-circ-ARAP2) or negative control (si-NC). Data are presented as the mean ± SD. ^***p < 0.001 vs. control. B, C CCK8 detection show the effect of circ-ARAP2 to proliferation of KYSE-150R and ECA-109R. Data are presented as the mean ± SD. ^***p < 0.001 vs. si-NC. D, E Cloning formation assay showing the cellular proliferation in both KYSE-150R and ECA-109R cells. Data are presented as the mean ± SD. ^***p < 0.001 vs. si-NC. F–H Representative photographs of KYSE-150R tumor formation in the xenografts of nude mice.Summary of the tumor volume and in mice that were measured weekly. Data are presented as the mean ± SD. **p < 0.01; ***p < 0.001 vs. si-nc. Tumor weight was measured 30 days post-injection. Data are presented as the mean ± sd. ***p < 0.001 vs. sh-NC. I, J Immunohistochemical analysis shows the percentage of Ki-67-positive cells. Data are presented as the mean ± SD. ***P < 0.001 vs. sh-NC