Fig. 5

Silencing eIF6 expression inhibited the proliferation of ESCA cells. A Western blot analysis of eIF6 protein in normal epithelial cells (HET1A) and different ESCA cell lines. B qRT-PCR and Western blot analysis confirming the success of eIF6-siRNAs transfection. C Silencing of eIF6 expression reduced the growth of Eca109 and KYSE30 cells as revealed by MTS assays at 24, 48, and 72 h after transfection. D Cell proliferation decreased after eIF6-siRNAs treatment as detected by EdU staining. *p < 0.05, **p < 0.01, ***p < 0.001