Fig. 2

Functional analysis of the genes correlated or regulated by eIF6 in TCGA database. A A heat map of the top 10 genes correlated with eIF6 in the TCGA-ESCA patients. B Bubble diagrams showing the enrichment results of the top 200 genes correlated with eIF6 in ESCA. C The top 10 hub genes with the most connected degrees determined using the cytoHubba plugin in Cytoscape. D Volcano plots displaying the differentially expressed genes correlated with eIF6. E The HALLMARK pathways and glycolysis signatures that were significantly in patients with high or low eIF6 expression as determined with GSEA. F Venn diagram of the glycolytic genes that significantly correlated with eIF6 in TIMER database and TCGA database, and differentially expressed in high and low eIF6 expressing ESCA groups. G The expression of eight glycolytic genes identified from the Venn diagram in tumor tissues and normal tissues in TCGA-ESCA database. *p < 0.05, **p < 0.01, ***p < 0.001. H Overall survival curve of patients with ESCC according to their GPC1 or GOT1 expression in Kaplan–Meier plot database