Fig. 1
The establishment of the HTRF high thought put screening system based on the combination of ACE2 and S-RBD. A The selection of the tag antibody. The Fc-d2 and His-Tb pair can lead to the highest signal ratio at the same concentration of ACE and S-RBD. B The optimization of substrate concentration. The combination of ACE2-Fc at 1.15 μg/ml and S-RBD at 0.88 μg/ml can reach the highest signal ratio. C There is no significant change of the signal with the time. D Nafamostat mesylate was selected as positive control. The EC50 of Nafamostat mesylate was 11.34 μM. E The verification of the high through put system show that the Z factor was 0.67 which was good enough for the high though put screening