Fig. 2From: The methyltransferase METTL3 promotes tumorigenesis via mediating HHLA2 mRNA m6A modification in human renal cell carcinomaDepletion of METTL3 expression in ccRCC cell lines. The stable depletion model of METTL3 in ccRCC cell lines 786-O and ACHN was established by using the RNAi method. A qRT-PCR results showed that the expression of METTL3 at the mRNA level was significantly decreased in LV-METTL3-shRNA compared with LV-NC in both 786-O and ACHN cells (P < 0.05 respectively). B and C Western blot analysis showed that METTL3 expression protein level was significantly decreased in LV-METTL3-shRNA compared with LV-NC in both 786-O and ACHN cells (P < 0.05 respectively). D CCK-8 assay showed that the cell proliferation ability of LV-METTL3-shRNA was significantly inhibited compared with the LV-NC (P < 0.05 in 786-O cells at 72 h, P < 0.05 at 48 h and P < 0.01 at 72 h respectively in ACHN cells). E and F Transwell assay results revealed that the cell invasion ability of LV-METTL3-shRNA was significantly decreased compared with LV-NC (P < 0.05 in 786-O cells, and P < 0.05 in ACHN cells). G and H Wound-healing assay showed that at the time point of 24 h, the relative wound closure rate of LV-METTL3-shRNA was significantly decreased compared with LV-NC (P < 0.05 in 786-O and P < 0.01 in ACHN cells, respectively). Un-paired t test was used as needed. *P < 0.05, **P < 0.01Back to article page