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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: Exosomes derived from myeloid-derived suppressor cells facilitate castration-resistant prostate cancer progression via S100A9/circMID1/miR-506-3p/MID1

Fig. 3

Exosomal S100A9 from MDSC promoted circMID1 expression in PC3 cells. A A representative western blot of S100A9 expression in MDSC-Exo, and quantitative analysis of S100A9 expression. GAPDH is control protein for cells, Hsp70 is control protein for exosomes. **P < 0.01, NS: no significance. B A representative western blot of S100A9 expression in MDSCs and MDSC-Exo treated with control siRNA (si-NC) or S100A9 siRNA (si-S100A9), and quantitative analysis of S100A9 expression. **P < 0.01, * vs. si-NC. C Relative expression of circMID1 in PC3 and DU145 cells treated with MDSC-Exo or MDSC-Exosi-S100A9. **, ## P < 0.01, *vs. control, # vs. Exo. D The expressions of RNA-binding protein DHX9 and Quaking (QKI) were determined by western blotting in PC3 and DU145 cells treated with MDSC-Exo or MDSC-Exosi-S100A9. The analysis of result of western blot for DHX9 and QKI. **, ##P < 0.01, *vs. control, # vs. Exo. E The distribution of IARs within the MID1 gene locus. The upper line shows the whole length of MID1, while the lower line shows ± 500 bp of the region between exon 7 and exon 8. F The expression levels of DHX9 were evaluated by western blotting after transfection with DHX9 siRNAs in PC3 and DU145 cells, and quantitative analysis of S100A9 expression. **P < 0.01, * vs. si-NC. G The relative levels of circMID1 in MDSC-Exo-treated PC3 and DU145 cells were measured after DHX9 was silenced. *, #P < 0.05; **, ##P < 0.01, * vs. control + si-NC. H The uptake of MDSC-derived exosomes by PC3 and DU145 cells at 48 h. The free CFSE treated cells was used as a control (Control). Green, CFSE-labeled exosomes; red, alpha-tubulin, a microtubule marker (scale bar, 20 μm). I The level of S100A9 in PC3 and DU145 cells after 48-h incubation with MDSC-Exo was determined by western blotting. The analysis of result of western blot for S100A9. *P < 0.05

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