Fig. 8

Effects of GANT and AFA, alone or in combination, on the Hh pathway. A Real-time q-PCR was performed to assess basal levels of Hh pathway activity in MM cell lines. B GLI1, GLI2, and Ptch1 mRNA levels in MM cell lines treated with AFA 10 μM, GANT 20 μM, AFA 10 μM + GANT 20 μM, or DMSO for 24 h. GLI1, GLI2, and Ptch1 mRNA expression levels are shown after normalization to the controls. The results are expressed as the mean ± SD of three independent experiments. Statistical significance of the effects obtained with GANT and AFA, alone or in combination, was calculated vs those obtained in (1) DMSO-, (2) GANT-, and (3) AFA-treated cells (˟p ≤ 0.05, *p ≤ 0.005, ^#p ≤ 0.0005) with a two-tailed Student’s t test