Fig. 5

Effects of GANT and AFA, alone or in combination, on molecules involved in apoptosis in MM cell lines. A The expression of Bax, Bcl-2, caspase 8, caspase 3, PARP-1 and γH2AX was evaluated by Western blotting analysis following treatment for 48 h with AFA 10 μM, GANT 20 μM, AFA 10 μM + GANT 20 μM, or DMSO. Actin was used as internal control. B The densitometric ratios between γH2AX and actin, Bax and Bcl-2, and statistical analysis are reported. The intensity of the bands was quantified using the ImageJ 1.53e software after blot scanning. Statistical significance of the effects obtained with GANT and AFA, alone or in combination, was calculated vs those obtained in (1) DMSO-, (2) GANT- and (3) AFA-treated cells with one-way ANOVA (˟p ≤ 0.05, *p ≤ 0.01, ^#p ≤ 0.001). Data are expressed as the mean ± SD of two independent experiments. Cropped images were used for Western Blotting