Fig. 5

Senescent EAT derived LEVs repress NMVMs Rictor expression and cause NMVMs dysfunction via miRNA-326-3p. A Western blotting analysis of Rictor, p-AKT, AKT and Gapdh in Con- or STZ-EAT LEVs co-cultured NMVMs transfected with miR decoy (Inh-326-3p) or negative decoy control (Inh-NC) (n = 3 per group). Con- or STZ-EAT LEVs co-cultured NMVMs were transfected with Inh-326-3p or Inh-NC and B contractile function (n = 6 per group), C mitochondrial membrane potential (TMRM) and mitochondrial superoxide (MitoSox) (n = 5 per group), and D mitochondrial respiration (n = 6 per group) were assayed. Data are presented as the mean ± SEM; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared to STZ-EAT LEVs group; ^#P < 0.05, ^##P < 0.01 compared to Inh-NC group