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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Secretion of miRNA-326-3p by senescent adipose exacerbates myocardial metabolism in diabetic mice

Fig. 2

LEVs isolated from senescent EAT in diabetic mice drives contractile and mitochondrial dysfunction in NMVMs. A Representative transmission electron micrograph of isolated LEVs. B Representative micrograph of EAT derived LEVs (PKH67-labeled, green) co-cultured with NMVMs. C Contractile velocity of untreated (UT) or NMVMs treated with Con-EAT LEVs or STZ-EAT LEVs (n = 6 per group). D Representative micrograph and E quantification of untreated (UT) and NMVMs treated with Con-EAT LEVs or STZ-EAT LEVs stained for mitochondrial membrane potential (TMRM), mitochondrial superoxide (MitoSox), and DAPI (n = 5 per group). F Real-time oxygen consumption rates (OCR) were evaluated for untreated (UT) or NMVMs treated with Con-EAT LEVs or STZ-EAT LEVs; basal and maximal respiration rates are shown (n = 8–9 per group). G Percentage of SA-β-gal positive cells in STZ-EAT cultured ex vivo in presence of Seno or Veh (n = 3 per group). H Relative gene expression of senescence associated genes in STZ-EAT cultured ex vivo in presence of Seno or Veh. (n = 3 per group). I Contractile velocity, J mitochondrial membrane potential and mitochondrial superoxide relative intensity fluorescence of NMVMs treated with LEVs from STZ-EAT cultured ex vivo in presence of Seno or Veh (n = 5–6 per group). (K) Real-time oxygen consumption rates (OCR) were evaluated for NMVMs treated with LEVs from STZ-EAT cultured ex vivo in presence of Seno or Veh, basal and maximal respiration rates are shown (n = 8–9 per group). Data are presented as the mean ± SEM; *P < 0.05, ***P < 0.001, ****P < 0.0001 compared to UT group; ##P < 0.01, ###P < 0.001, ####P < 0.0001 compared to Con-EAT LEVs group; $P < 0.05, $$P < 0.01, $$$P < 0.001 compared to Veh-STZ-EAT LEVs group

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